Delivery of cytoplasmic and apoplastic effectors from Phytophthora infestans haustoria by distinct secretion pathways.
Identifieur interne : 000A34 ( Main/Exploration ); précédent : 000A33; suivant : 000A35Delivery of cytoplasmic and apoplastic effectors from Phytophthora infestans haustoria by distinct secretion pathways.
Auteurs : Shumei Wang [Royaume-Uni] ; Petra C. Boevink [Royaume-Uni] ; Lydia Welsh [Royaume-Uni] ; Ruofang Zhang [République populaire de Chine] ; Stephen C. Whisson [Royaume-Uni] ; Paul R J. Birch [Royaume-Uni]Source :
- The New phytologist [ 1469-8137 ] ; 2017.
Descripteurs français
- KwdFr :
- Bréfeldine A (pharmacologie), Cellules végétales (effets des médicaments et des substances chimiques), Cellules végétales (microbiologie), Cellules végétales (métabolisme), Cytoplasme (effets des médicaments et des substances chimiques), Cytoplasme (métabolisme), Noyau de la cellule (effets des médicaments et des substances chimiques), Noyau de la cellule (métabolisme), Phytophthora infestans (effets des médicaments et des substances chimiques), Phytophthora infestans (métabolisme), Protéines de fusion recombinantes (métabolisme), Redistribution de fluorescence après photoblanchiment (MeSH), Tabac (microbiologie), Voie de sécrétion (effets des médicaments et des substances chimiques).
- MESH :
- effets des médicaments et des substances chimiques : Cellules végétales, Cytoplasme, Noyau de la cellule, Phytophthora infestans, Voie de sécrétion.
- microbiologie : Cellules végétales, Tabac.
- métabolisme : Cellules végétales, Cytoplasme, Noyau de la cellule, Phytophthora infestans, Protéines de fusion recombinantes.
- pharmacologie : Bréfeldine A.
- Redistribution de fluorescence après photoblanchiment.
English descriptors
- KwdEn :
- Brefeldin A (pharmacology), Cell Nucleus (drug effects), Cell Nucleus (metabolism), Cytoplasm (drug effects), Cytoplasm (metabolism), Fluorescence Recovery After Photobleaching (MeSH), Phytophthora infestans (drug effects), Phytophthora infestans (metabolism), Plant Cells (drug effects), Plant Cells (metabolism), Plant Cells (microbiology), Recombinant Fusion Proteins (metabolism), Secretory Pathway (drug effects), Tobacco (microbiology).
- MESH :
- chemical , metabolism : Recombinant Fusion Proteins.
- chemical , pharmacology : Brefeldin A.
- drug effects : Cell Nucleus, Cytoplasm, Phytophthora infestans, Plant Cells, Secretory Pathway.
- metabolism : Cell Nucleus, Cytoplasm, Phytophthora infestans, Plant Cells.
- microbiology : Plant Cells, Tobacco.
- Fluorescence Recovery After Photobleaching.
Abstract
The potato blight pathogen Phytophthora infestans secretes effector proteins that are delivered inside (cytoplasmic) or can act outside (apoplastic) plant cells to neutralize host immunity. Little is known about how and where effectors are secreted during infection, yet such knowledge is essential to understand and combat crop disease. We used transient Agrobacterium tumefaciens-mediated in planta expression, transformation of P. infestans with fluorescent protein fusions and confocal microscopy to investigate delivery of effectors to plant cells during infection. The cytoplasmic effector Pi04314, expressed as a monomeric red fluorescent protein (mRFP) fusion protein with a signal peptide to secrete it from plant cells, did not passively re-enter the cells upon secretion. However, Pi04314-mRFP expressed in P. infestans was translocated from haustoria, which form intimate interactions with plant cells, to accumulate at its sites of action in the host nucleus. The well-characterized apoplastic effector EPIC1, a cysteine protease inhibitor, was also secreted from haustoria. EPIC1 secretion was inhibited by brefeldin A (BFA), demonstrating that it is delivered by conventional Golgi-mediated secretion. By contrast, Pi04314 secretion was insensitive to BFA treatment, indicating that the cytoplasmic effector follows an alternative route for delivery into plant cells. Phytophthora infestans haustoria are thus sites for delivery of both apoplastic and cytoplasmic effectors during infection, following distinct secretion pathways.
DOI: 10.1111/nph.14696
PubMed: 28758684
PubMed Central: PMC5601276
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Little is known about how and where effectors are secreted during infection, yet such knowledge is essential to understand and combat crop disease. We used transient Agrobacterium tumefaciens-mediated in planta expression, transformation of P. infestans with fluorescent protein fusions and confocal microscopy to investigate delivery of effectors to plant cells during infection. The cytoplasmic effector Pi04314, expressed as a monomeric red fluorescent protein (mRFP) fusion protein with a signal peptide to secrete it from plant cells, did not passively re-enter the cells upon secretion. However, Pi04314-mRFP expressed in P. infestans was translocated from haustoria, which form intimate interactions with plant cells, to accumulate at its sites of action in the host nucleus. The well-characterized apoplastic effector EPIC1, a cysteine protease inhibitor, was also secreted from haustoria. EPIC1 secretion was inhibited by brefeldin A (BFA), demonstrating that it is delivered by conventional Golgi-mediated secretion. By contrast, Pi04314 secretion was insensitive to BFA treatment, indicating that the cytoplasmic effector follows an alternative route for delivery into plant cells. Phytophthora infestans haustoria are thus sites for delivery of both apoplastic and cytoplasmic effectors during infection, following distinct secretion pathways.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28758684</PMID><DateCompleted><Year>2018</Year><Month>05</Month><Day>15</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><Volume>216</Volume><Issue>1</Issue><PubDate><Year>2017</Year><Month>Oct</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>Delivery of cytoplasmic and apoplastic effectors from Phytophthora infestans haustoria by distinct secretion pathways.</ArticleTitle><Pagination><MedlinePgn>205-215</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/nph.14696</ELocationID><Abstract><AbstractText>The potato blight pathogen Phytophthora infestans secretes effector proteins that are delivered inside (cytoplasmic) or can act outside (apoplastic) plant cells to neutralize host immunity. Little is known about how and where effectors are secreted during infection, yet such knowledge is essential to understand and combat crop disease. We used transient Agrobacterium tumefaciens-mediated in planta expression, transformation of P. infestans with fluorescent protein fusions and confocal microscopy to investigate delivery of effectors to plant cells during infection. The cytoplasmic effector Pi04314, expressed as a monomeric red fluorescent protein (mRFP) fusion protein with a signal peptide to secrete it from plant cells, did not passively re-enter the cells upon secretion. However, Pi04314-mRFP expressed in P. infestans was translocated from haustoria, which form intimate interactions with plant cells, to accumulate at its sites of action in the host nucleus. The well-characterized apoplastic effector EPIC1, a cysteine protease inhibitor, was also secreted from haustoria. EPIC1 secretion was inhibited by brefeldin A (BFA), demonstrating that it is delivered by conventional Golgi-mediated secretion. By contrast, Pi04314 secretion was insensitive to BFA treatment, indicating that the cytoplasmic effector follows an alternative route for delivery into plant cells. Phytophthora infestans haustoria are thus sites for delivery of both apoplastic and cytoplasmic effectors during infection, following distinct secretion pathways.</AbstractText><CopyrightInformation>© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Shumei</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Division of Plant Sciences, University of Dundee (at JHI), Errol Road, Invergowrie, Dundee, DD2 5DA, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boevink</LastName><ForeName>Petra C</ForeName><Initials>PC</Initials><AffiliationInfo><Affiliation>Cell and Molecular Sciences, James Hutton Institute, Errol Road, Invergowrie, Dundee, DD2 5DA, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Welsh</LastName><ForeName>Lydia</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Cell and Molecular Sciences, James Hutton Institute, Errol Road, Invergowrie, Dundee, DD2 5DA, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Ruofang</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Potato Engineering and Technology Research Centre of Inner Mongolia University, West College Road 235, Hohhot, 010021, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Whisson</LastName><ForeName>Stephen C</ForeName><Initials>SC</Initials><AffiliationInfo><Affiliation>Cell and Molecular Sciences, James Hutton Institute, Errol Road, Invergowrie, Dundee, DD2 5DA, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Birch</LastName><ForeName>Paul R J</ForeName><Initials>PRJ</Initials><AffiliationInfo><Affiliation>Division of Plant Sciences, University of Dundee (at JHI), Errol Road, Invergowrie, Dundee, DD2 5DA, UK.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Cell and Molecular Sciences, James Hutton Institute, Errol Road, Invergowrie, Dundee, DD2 5DA, UK.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>BB/G015244/1</GrantID><Agency>Biotechnology and Biological Sciences Research Council</Agency><Country>United Kingdom</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>07</Month><Day>31</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>20350-15-6</RegistryNumber><NameOfSubstance UI="D020126">Brefeldin A</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="CommentIn"><RefSource>New Phytol. 2017 Oct;216(1):8-10</RefSource><PMID Version="1">28850183</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D020126" MajorTopicYN="N">Brefeldin A</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002467" MajorTopicYN="N">Cell Nucleus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003593" MajorTopicYN="N">Cytoplasm</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D036681" MajorTopicYN="N">Fluorescence Recovery After Photobleaching</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059828" MajorTopicYN="N">Plant Cells</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055571" MajorTopicYN="Y">Secretory Pathway</DescriptorName><QualifierName UI="Q000187" 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last="Wang">Shumei Wang</name></noRegion><name sortKey="Birch, Paul R J" sort="Birch, Paul R J" uniqKey="Birch P" first="Paul R J" last="Birch">Paul R J. Birch</name><name sortKey="Birch, Paul R J" sort="Birch, Paul R J" uniqKey="Birch P" first="Paul R J" last="Birch">Paul R J. Birch</name><name sortKey="Boevink, Petra C" sort="Boevink, Petra C" uniqKey="Boevink P" first="Petra C" last="Boevink">Petra C. Boevink</name><name sortKey="Welsh, Lydia" sort="Welsh, Lydia" uniqKey="Welsh L" first="Lydia" last="Welsh">Lydia Welsh</name><name sortKey="Whisson, Stephen C" sort="Whisson, Stephen C" uniqKey="Whisson S" first="Stephen C" last="Whisson">Stephen C. Whisson</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Ruofang" sort="Zhang, Ruofang" uniqKey="Zhang R" first="Ruofang" last="Zhang">Ruofang Zhang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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